MGM Core

Mitochondrial dysfunction may represent one of the key cellular pathologies in Alzheimer’s disease and may be intimately related to its progression. Defective metabolism and increased generation of excess reactive oxygen species in mitochondria, as widely observed in Alzheimer's cases, can lead to mitochondrial DNA (mtDNA) oxidative modification and likely increases in rates of mtDNA mutations. Such molecular changes can enhance the mitochondrial and cellular pathology, especially in populations of neurons selectively vulnerable to oxidative stress.

The Mitochondrial Genomics and Metabolism (MGM) Core of the KU Azheimer's Disease Center was structured to provide unique resources and expertise to investigators who plan to probe mitochondrial changes in Alzheimer's disease and unravel the relationships between Alzheimer's and altered mitochondrial metabolism in white blood cells, platelets, neurons, glia, and muscle cells.

Core Resources and Expertise of MGM

MGM will assist investigators in studies on mitochondria obtained from the brain, muscle, white blood cells, and platelets of well-characterized cases of Alzheimer's, mild cognitive impairment (MCI), and age-matched controls.  Specifically, MGM offers the following core resources and expertise for ADC investigators:

  • Preparation, cataloging, and storage of mitochondria, protein extracts, DNA, and RNA from living and deceased subjects recruited by the Clinical Core
  • Preparation and banking of cybrid lines using neuronal and platelet mitochondria obtained from living subjects
  • Sequencing of mtDNA, determination of levels of mtDNA heteroplasmy, and measurements of mtDNA oxidation

Goals

The goal of the MGM core is to jumpstart independently funded research focused on Alzheimer's disease and mtDNA gene and protein structure, function, and expression. The ultimate goal is to enhance Alzheimer's disease related research into mitochondrial structure and function in the Kansas City region and assist national research efforts focused on mitochondria. MGM’s expertise in mitochondrial genome sequencing, heteroplasmy determination, mtDNA oxidation, cybrid generation, and mitochondrial metabolic activity measurements can prove to be an invaluable resource for investigators interested in the role of mitochondrial function and Alzheimer's.

Last modified: Sep 25, 2012
Requesting resources
brain puzzle

MGM's core resources, including the preparation of mitochondria, cybrid lines, DNA, RNA, and protein extracts, are available upon request.For more information or to request service, please contact MGM@ku.edu.

A project description, possible available funding, and approaches to future funding of the project will be required before commitment of MGM and KU Alzheimer's Disease Center resources to any individual project.

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